Oversampling method to extract excitatory and
inhibitory conductances from single-trial
membrane potential recordings.
Claude Bédard, Sébastien Béhuret, Charlotte
Deleuze, Thierry Bal and Alain Destexhe
J. Neurosci. Methods, in press, 2011.
Abstract
Variations of excitatory and inhibitory conductances determine
the membrane potential (Vm) activity of neurons, as well as their
spike responses, and are thus of primary importance. Methods to
estimate these conductances require clamping the cell at several
different levels of Vm, thus making it impossible to estimate
conductances from "single trial'' Vm recordings. We present here a
new method that allows extracting estimates of the time course
excitatory and inhibitory conductances from single-trace Vm
recordings. This method is based on oversampling of the Vm. We
test the method numerically using models of increasing complexity.
Finally, the method is evaluated using controlled conductance
injection in cortical neurons in vitro using the dynamic-clamp
technique. This conductance extraction method should be very
useful for future in vivo applications.
MATLAB Demo
This MATLAB code implements the method of conductance
extraction developed here, and also reproduces several figures of
the paper. The MATLAB code was written by Claude Bedard.
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